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INSTITUT DE BIOLOGIE DE L'ÉCOLE NORMALE SUPÉRIEURE

Equipement

The genomic platform of the IBENS brings together all devices needed for high-throughput sequencing of functional genomic projects. The machines are accessible to any laboratory or private company who wish to use them. There is a free access for the less sensitive machines. For the other devices, more sophisticated, they can be used within a collaboration project or after a training session.

 

 

How to book the platform devices

To manage access to the self-service devices on the platform, we provide a booking calendar.

To go on the calendar page click here or use the menu on your right.

High throughput analyses

High-throughput sequencer – NextSeq 500

  • Manufacturer: Illumina
  • Installation: April 2015
  • Sequencing of up to 400 million parallel reads. Two sequencing modes: high or mid output. From 75bp to 2x150bp reads.
  • Access: only through the platform staff.

qPCR à Haut Débit – Biomark HD system

  • Manufacturer: Fluidigm
  • Installation: April 2013
  • High-throughput quantitative PCR system using microfluidics.
  • Access: contact the qPCR-HD platform qpcr@biologie.ens.fr or go on their web site.

Lecteur d’absorbance en plaque – SpectraMax Plus384

  • Manufacturer: Molecular Devices
  • Installation: April 2000
  • Quantification of PCR products and others.
  • Access: free access (booking required) after training by platform staff.

Sample preparation

Système de préparation de banques Apollo 324

  • Manufacturer: Wafergen
  • Installation: January 2014
  • RNA library preparation using polyA purification, ribosomal depletion or amplification for small material amounts. Flexible system allowing to prepare from 2 to 48 librairies at a time.
  • Access: only through the platform staff.

Ultra-sonicateur – S220

  • Manufacturer: Covaris
  • Installation: June 2013
  • Sonication of the samples with a targeted wave that ensures homogeneity and no heating. Allows fragmentation of libraries for high-throughput sequencing and fragmentation of DNA samples for Immuno Precipitation experiments.
  • Access: free access (booking required) after training by platform staff. Consumables are at the user’s expense.

Système microfluidique de préparation des banques – Mondrian SP+

  • Manufacturer: NuGEN
  • Installation: April 2013
  • Fully automated library preparation for DNA and RNA, with a microfluidic system.
  • Access: only through the platform staff.

Automate pour la manipulation de liquide – BioRobot 8000

  • Manufacturer: Qiagen
  • Installation: March 2001
  • Robot for liquid handling, colonies pricking out and collection rearrangements. One arm with a eight-channel head: variable gap, volume detection system and bar code reader. The robot performs DNA extractions and agarose gel drop-offs.
  • Access: only through the platform staff.

Quality control

Électrophorèse capillaire – Bioanalyzer 2100

  • Manufacturer: Agilent
  • Installation: January 2002
  • Quality control of DNA and RNA sample. Very quick and using only small volumes.
  • Access: free access (booking required) after training by platform staff. Consumables are at the user’s expense.

Spectrophotomètre à UV – Nanodrop ND-1000

  • Manufacturer: NanoDrop Technologies
  • Installation: March 2006
  • Quality control of DNA and RNA sample. Very quick and using only small volumes.
  • Access: free access (booking required)..

Fluorimètre – QuBit 2.0

  • Manufacturer: Life Technologies
  • Installation: May 2012
  • Quantification of DNA, RNA and protein samples (high sensitivity).
  • Access: free access.