Equipement

The GenomiqueENS core facility brings together all devices needed for high-throughput sequencing of functional genomic projects. The machines are accessible to any laboratory or private company who wish to use them. There is a free access for the less sensitive machines. For the other devices, more sophisticated, they can be used within a collaboration project or after a training session.

How to book the platform devices

To manage access to the self-service devices on the platform, we provide a booking calendar.

To go on the calendar page click here or use the menu on your right.

High throughput analyses

High-throughput sequencer – NextSeq 2000 Manufacturer: Illumina Installation: June 2021 Sequencing of up to 1.1 billion parallel reads. From 50bp to 2x150bp reads. Access: only through the platform staff.
High-throughput sequencer – Mk1C Manufacturer: Oxford Nanopore Technologies Installation: August 2020 Long read RNA-seq. Access: only through the platform staff.
High-throughput sequencer – NextSeq 500 Manufacturer: Illumina Installation: April 2015 Sequencing of up to 400 million parallel reads. Two sequencing modes: high or mid output. From 75bp to 2x150bp reads. Access: only through the platform staff.
High-throughput sequencer – MinION Manufacturer: Oxford Nanopore Technologies Installation: May 2016 Long read RNA-seq. Access: only through the platform staff.
Single Cell System – Chromium Manufacturer: 10X Genomics Installation: December 2017 Can isolate until 10,000 cells in droplets to allow mRNA to be sequenced. Access: only through the platform staff.
High-throughput qPCR – Biomark HD system Manufacturer: Fluidigm Installation: April 2013 High-throughput quantitative PCR system using microfluidics. Access: contact the qPCR-HD platform qpcr@biologie.ens.fr or go on their web site.
Microplate reader – SpectraMax Plus384 Manufacturer: Molecular Devices Installation: April 2000 Quantification of PCR products and others. Access: free access (booking required) after training by platform staff.

Sample preparation

Ultrasonicator – S220 Manufacturer: Covaris Installation: June 2013 Sonication of the samples with a targeted wave that ensures homogeneity and no heating. Allows fragmentation of libraries for high-throughput sequencing and fragmentation of DNA samples for Immuno Precipitation experiments. Access: free access (booking required) after training by platform staff. Consumables are at the user’s expense.

Quality control

Capillary electrophoresis – Fragment Analyzer Manufacturer: Agilent / Advanced Analytical Model: 12 capillary Installation: January 2018 Quality control of DNA and RNA samples. Using small volumes up to 96 samples with 3 plates positions. Access: free access (booking required) after training by platform staff. Contact us for prices.
UV Spectrophotometer – Nanodrop ONEC Manufacturer: ThermoFisher Installation: December 2021 Quality control of DNA and RNA sample. Very quick and using only small volumes. Access: free access (booking required)..
UV Spectrophotometer – Nanodrop ND-1000 Manufacturer: NanoDrop Technologies Installation: March 2006 Quality control of DNA and RNA sample. Very quick and using only small volumes. Access: free access (booking required)..
Fluorimeter – QuBit 3.0 Manufacturer: Life Technologies Installation: May 2017 Quantification of DNA, RNA and protein samples (high sensitivity). Access: free access.