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INSTITUT DE BIOLOGIE DE L'ÉCOLE NORMALE SUPÉRIEURE

Equipement

The GenomiqueENS core facility brings together all devices needed for high-throughput sequencing of functional genomic projects. The machines are accessible to any laboratory or private company who wish to use them. There is a free access for the less sensitive machines. For the other devices, more sophisticated, they can be used within a collaboration project or after a training session.

 

 

How to book the platform devices

To manage access to the self-service devices on the platform, we provide a booking calendar.

To go on the calendar page click here or use the menu on your right.

High throughput analyses

High-throughput sequencer – NextSeq 2000

  • Manufacturer: Illumina
  • Installation: June 2021
  • Sequencing of up to 1.1 billion parallel reads. From 50bp to 2x150bp reads.
  • Access: only through the platform staff.

High-throughput sequencer – PromethION P2 solo

  • Manufacturer: Oxford Nanopore Technologies
  • Installation: December 2022
  • Long read RNA-seq.
  • Access: only through the platform staff.

High-throughput sequencer – MinION Mk1B

  • Manufacturer: Oxford Nanopore Technologies
  • Installation: May 2016
  • Long read RNA-seq.
  • Access: only through the platform staff.

Single Cell System – Chromium X/iX

  • Manufacturer: 10X Genomics
  • Installation: November 2023
  • Can isolate until 10,000 cells in droplets to allow mRNA to be sequenced.
  • Access: only through the platform staff.

Single Cell System – Chromium

  • Manufacturer: 10X Genomics
  • Installation: December 2017
  • Can isolate until 10,000 cells in droplets to allow mRNA to be sequenced.
  • Access: only through the platform staff.

Microplate reader – SpectraMax Plus384

  • Manufacturer: Molecular Devices
  • Installation: April 2000
  • Quantification of PCR products and others.
  • Access: free access (booking required) after training by platform staff.

Sample preparation

Ultrasonicator – S220

  • Manufacturer: Covaris
  • Installation: June 2013
  • Sonication of the samples with a targeted wave that ensures homogeneity and no heating. Allows fragmentation of libraries for high-throughput sequencing and fragmentation of DNA samples for Immuno Precipitation experiments.
  • Access: free access (booking required) after training by platform staff. Consumables are at the user’s expense.

Quality control

Capillary electrophoresis – Fragment Analyzer

  • Manufacturer: Agilent / Advanced Analytical
  • Model: 12 capillary
  • Installation: January 2018
  • Quality control of DNA and RNA samples. Using small volumes up to 96 samples with 3 plates positions.
  • Access: free access (booking required) after training by platform staff. Contact us for prices.

UV Spectrophotometer – Nanodrop ONEC

  • Manufacturer: ThermoFisher
  • Installation: December 2021
  • Quality control of DNA and RNA sample. Very quick and using only small volumes.
  • Access: free access (booking required)..

UV Spectrophotometer – Nanodrop ND-1000

  • Manufacturer: NanoDrop Technologies
  • Installation: March 2006
  • Quality control of DNA and RNA sample. Very quick and using only small volumes.
  • Access: free access (booking required)..

Fluorimeter – QuBit 3.0

  • Manufacturer: Life Technologies
  • Installation: May 2017
  • Quantification of DNA, RNA and protein samples (high sensitivity).
  • Access: free access.